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KEY RESOURCES TABLE

Journal: Cell systems

Article Title: Design and construction of generalizable RNA-protein hybrid controllers by level-matched genetic signal amplification

doi: 10.1016/j.cels.2016.10.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Theophylline Sigma-Aldrich LOT# 091M0214V Neomycin (as fradiomycin sulfate) TCI America LOT# SCFUG-KI Tetracycline Sigma-Aldrich LOT# BCBK1604V (6 R )-folinic acid Schircks Laboratories LOT# 154 Caffeine Sigma-Aldrich C0750 Caffeine-(trimedthyl-13C3) Sigma-Aldrich 485365 Critical Commercial Assays QIAquick PCR Purification kit QIAGEN 28106 MEGAshortscript T7 kit Life Technologies AM1354 RNA Clean & Concentrator kit Zymo Research R1019 DNA Clean & Concentrator kit Zymo Research D4014 EconoSpin Mini Spin Column Epoch Life Science 1910-050/250 Experimental Models: Organisms/Strains S. cerevisiae: Strain background W303 ATCC ATTC: 208353 S. cerevisiae: Device-harboring strains This paper Table S1 and Supplemental Data 8 E. coli: TOP10 Thermo Fisher Scientific C404010 Recombinant DNA Plasmids This paper Supplemental Data 8 Sequence-Based Reagents DNA primers This paper Supplemental Data 8 Ribozyme-based RNA switches, and ribozymes This paper Supplemental Data 8 DNA and protein parts This paper Supplemental Data 8 Software and Algorithms MATLAB (Windows ver R2013b) MATLAB www.mathworks.com/products/matlab Prism (Windows ver.

Techniques: Recombinant, Purification, Sequencing, Software